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length pparγ2 mammalian expression construct  (Sino Biological)


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    Sino Biological length pparγ2 mammalian expression construct
    Length Pparγ2 Mammalian Expression Construct, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/length+ppar%CE%B32/Human+PPARG+Gene+ORF+cDNA+clone+expression+plasmid%2C+N-HA+tag/pm40388619-256-64-69
    Average 94 stars, based on 1 article reviews
    length pparγ2 mammalian expression construct - by Bioz Stars, 2026-09
    94/100 stars

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    Transfection:

    Article Title: A tethering mechanism underlies Pin1-catalyzed proline cis-trans isomerization at a noncanonical site
    Article Snippet: HEK293T cells (ATCC, 12022001) were cultured in Dulbecco’s minimal essential medium (DMEM, Gibco 11960-044) supplemented with 10% fetal bovine serum (FBS, Gibco 26140-079) and Penicillin-Streptomycin-Glutamine (Gibco 10378-016) at 37°C in 5% CO2 atmosphere. .. 15,000 cells/well were plated in a 96 well plate (Greiner 655083) in 100 μL of media, left overnight, and transfected the following day with 50 ng PPRE, 25 ng PIN1, 25 ng N-terminal HA-tagged full length PPARγ2 (Sino Biological HG12019-NY), and 25 ng pCDNA3.1 empty vector in accordance with experimental conditions using Lipofectamine 3000 (Invitrogen L3000008) and OptiMEM (Gibco 31985-070). ..



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    Fig. 1 A, Sequence of PPRE in pGL3-Promoter vector used for reporter assay. Consensus sequence of PPAR responsive element (1xPPRE) was designed based on referenced publications. Shaded nucleo- tides indicate direct repeat sequences separated by a nucleotide. B, Effects of teneligliptin on PPRE-reporter activity. NIH3T3 cells were transfected with luciferase reporter construct pGL3-Promot- er-PPRE, under control of a SV40 promoter and 1xPPRE sequence and a <t>PPARγ2</t> overexpression construct. Teneligliptin (50 μM in culture medium) significantly transactivated luciferase reporter gene downstream of 1xPPRE. No significant increase was evident in negative control cells transfected with a reporter construct har- boring 1xPPRE but without PPARγ2 overexpression (1xPPRE, w/o PPARγ) or a reporter construct harboring no PPRE but with PPARγ2 overexpression (no PPRE, w PPARγ) at any tested dose of teneligliptin (n=8). *P<0.01.
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    Fig. 1 A, Sequence of PPRE in pGL3-Promoter vector used for reporter assay. Consensus sequence of PPAR responsive element (1xPPRE) was designed based on referenced publications. Shaded nucleo- tides indicate direct repeat sequences separated by a nucleotide. B, Effects of teneligliptin on PPRE-reporter activity. NIH3T3 cells were transfected with luciferase reporter construct pGL3-Promot- er-PPRE, under control of a SV40 promoter and 1xPPRE sequence and a <t>PPARγ2</t> overexpression construct. Teneligliptin (50 μM in culture medium) significantly transactivated luciferase reporter gene downstream of 1xPPRE. No significant increase was evident in negative control cells transfected with a reporter construct har- boring 1xPPRE but without PPARγ2 overexpression (1xPPRE, w/o PPARγ) or a reporter construct harboring no PPRE but with PPARγ2 overexpression (no PPRE, w PPARγ) at any tested dose of teneligliptin (n=8). *P<0.01.
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    Fig. 1 A, Sequence of PPRE in pGL3-Promoter vector used for reporter assay. Consensus sequence of PPAR responsive element (1xPPRE) was designed based on referenced publications. Shaded nucleo- tides indicate direct repeat sequences separated by a nucleotide. B, Effects of teneligliptin on PPRE-reporter activity. NIH3T3 cells were transfected with luciferase reporter construct pGL3-Promot- er-PPRE, under control of a SV40 promoter and 1xPPRE sequence and a <t>PPARγ2</t> overexpression construct. Teneligliptin (50 μM in culture medium) significantly transactivated luciferase reporter gene downstream of 1xPPRE. No significant increase was evident in negative control cells transfected with a reporter construct har- boring 1xPPRE but without PPARγ2 overexpression (1xPPRE, w/o PPARγ) or a reporter construct harboring no PPRE but with PPARγ2 overexpression (no PPRE, w PPARγ) at any tested dose of teneligliptin (n=8). *P<0.01.
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    Image Search Results


    Fig. 1 A, Sequence of PPRE in pGL3-Promoter vector used for reporter assay. Consensus sequence of PPAR responsive element (1xPPRE) was designed based on referenced publications. Shaded nucleo- tides indicate direct repeat sequences separated by a nucleotide. B, Effects of teneligliptin on PPRE-reporter activity. NIH3T3 cells were transfected with luciferase reporter construct pGL3-Promot- er-PPRE, under control of a SV40 promoter and 1xPPRE sequence and a PPARγ2 overexpression construct. Teneligliptin (50 μM in culture medium) significantly transactivated luciferase reporter gene downstream of 1xPPRE. No significant increase was evident in negative control cells transfected with a reporter construct har- boring 1xPPRE but without PPARγ2 overexpression (1xPPRE, w/o PPARγ) or a reporter construct harboring no PPRE but with PPARγ2 overexpression (no PPRE, w PPARγ) at any tested dose of teneligliptin (n=8). *P<0.01.

    Journal: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi

    Article Title: Evaluation of Teneligliptin Effects on Transcriptional Activity of PPARγ in Cell-Based Assays.

    doi: 10.1272/jnms.2018_85-15

    Figure Lengend Snippet: Fig. 1 A, Sequence of PPRE in pGL3-Promoter vector used for reporter assay. Consensus sequence of PPAR responsive element (1xPPRE) was designed based on referenced publications. Shaded nucleo- tides indicate direct repeat sequences separated by a nucleotide. B, Effects of teneligliptin on PPRE-reporter activity. NIH3T3 cells were transfected with luciferase reporter construct pGL3-Promot- er-PPRE, under control of a SV40 promoter and 1xPPRE sequence and a PPARγ2 overexpression construct. Teneligliptin (50 μM in culture medium) significantly transactivated luciferase reporter gene downstream of 1xPPRE. No significant increase was evident in negative control cells transfected with a reporter construct har- boring 1xPPRE but without PPARγ2 overexpression (1xPPRE, w/o PPARγ) or a reporter construct harboring no PPRE but with PPARγ2 overexpression (no PPRE, w PPARγ) at any tested dose of teneligliptin (n=8). *P<0.01.

    Article Snippet: We overexpressed PPARγ2 without an epitope tag using a cDNA clone (MC201042) harboring full-length mouse Pparγ2 cDNA in pCMV6-Kan/Neo (OriGene).

    Techniques: Sequencing, Plasmid Preparation, Reporter Assay, Activity Assay, Transfection, Luciferase, Construct, Control, Over Expression, Negative Control

    Fig. 2 A, Effects of transcription coregulators on transactivation of PPRE-reporter construct. A lucifer- ase reporter construct containing 1xPPRE was co-transfected with constructs that express PPARγ2 and transcription coregulators such as PGC1α or SRC1. Adding teneligliptin into culture medium at 10 or 50 μM of positive-control cells overexpressing only PPARγ2 resulted in a signifi- cant activation of reporter gene expression (pcDNA, empty vector), whereas suppression or no effect on transactivation was found in cells overexpressing either transcription coregulators (n=4). *P<0.01, **P<0.05. B, Effects of GW9662, PPARγ antagonist, on PPRE-reporter transactivation in- duced by teneligliptin. GW9662 (1―10 μM) did not affect PPRE-reporter transactivation induced by teneligliptin (10 and 50 μM) (n=8). *P<0.01.

    Journal: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi

    Article Title: Evaluation of Teneligliptin Effects on Transcriptional Activity of PPARγ in Cell-Based Assays.

    doi: 10.1272/jnms.2018_85-15

    Figure Lengend Snippet: Fig. 2 A, Effects of transcription coregulators on transactivation of PPRE-reporter construct. A lucifer- ase reporter construct containing 1xPPRE was co-transfected with constructs that express PPARγ2 and transcription coregulators such as PGC1α or SRC1. Adding teneligliptin into culture medium at 10 or 50 μM of positive-control cells overexpressing only PPARγ2 resulted in a signifi- cant activation of reporter gene expression (pcDNA, empty vector), whereas suppression or no effect on transactivation was found in cells overexpressing either transcription coregulators (n=4). *P<0.01, **P<0.05. B, Effects of GW9662, PPARγ antagonist, on PPRE-reporter transactivation in- duced by teneligliptin. GW9662 (1―10 μM) did not affect PPRE-reporter transactivation induced by teneligliptin (10 and 50 μM) (n=8). *P<0.01.

    Article Snippet: We overexpressed PPARγ2 without an epitope tag using a cDNA clone (MC201042) harboring full-length mouse Pparγ2 cDNA in pCMV6-Kan/Neo (OriGene).

    Techniques: Construct, Transfection, Positive Control, Activation Assay, Gene Expression, Plasmid Preparation